RIP Benny Harvey. Gone but not forgotten. Miss ya big man.
Sup, guise.
Submitted 1 week ago by fossilesque@mander.xyz to science_memes@mander.xyz
https://mander.xyz/pictrs/image/fb7a0c95-53de-4079-94e3-56e5debedec3.png
Comments
ordnance_qf_17_pounder@reddthat.com 1 week ago
Allero@lemmy.today 1 week ago
As someone who just borked a bacterial culture that is merely three months old, I can tell not all bacteria are made equal :D
tetris11@feddit.uk 1 week ago
whilst I approve of the use of Limmeh, I’m gonna need more context on the bacteria
Beryl@jlai.lu 1 week ago
You’d actually want to freeze them as fast as possible, to prevent the growth of large ice crystals that would tear the cell apart. That’s why you do it by dunking them in liquid nitrogen. But yeah frozen bacteria are basically immortal.
phdepressed@sh.itjust.works 1 week ago
No you add DMSO (5-10%) and freeze slowly. Using a Mr frosty or similar. Otherwise a few hours at -20, then -80, before the LN2.
Just chucking in LN2 is going to have terrible recovery. That might have been done with HeLa way back when but certainly isn’t standard anymore.
Beryl@jlai.lu 1 week ago
I mean obviously you’d use DMSO or glycerol, I just didn’t want to get too technical. That being said I’ve always snap-frozen bacteria with LN2 and it worked just fine. Now for eucaryotic cells, sure, you’d want to go slow.
Contramuffin@lemmy.world 1 week ago
You freeze mammalian cells by dunking them in LN2? I’ve… never heard of anyone do that. I’ve always put them in either a Mr. Frosty or a styrofoam conical holder (makeshift Mr. Frosty)
SydBa@sh.itjust.works 1 week ago
I really enjoy that the actual name for a scientific too is Mr. Frosty.
Beryl@jlai.lu 1 week ago
I guess I forgot about the first part of the meme and was talking bacteria, sorry. For eucaryotic cells sure you’d take your time in a -80°C first.