Comment on Finally back after a hiatus
ExcessShiv@lemmy.dbzer0.com 3 days agoconsumer freezers are a bit too leathal for yeast.
Is the frost-free freezer that are bad due to the freeze-thaw cycles that keeps the frost-free.
Comment on Finally back after a hiatus
ExcessShiv@lemmy.dbzer0.com 3 days agoconsumer freezers are a bit too leathal for yeast.
Is the frost-free freezer that are bad due to the freeze-thaw cycles that keeps the frost-free.
plactagonic@sopuli.xyz 3 days ago
I don’t remember that much, I think it is something about temperature - the -80°C freezers just freeze the culture much faster so you don’t kill the cells. You can mitigate it by using glycerol as antifreeze but it is bit more work.
With agar you store only the pure 1st gen of the yeasts so there is bit more work after you unfreeze them. So there is probably different method to freezing yeast cake.
When I messed with the yeast I used this playlist as instructions:
www.youtube.com/playlist?list=PLZ3Z2428mCTqDFK-3Z…
There is something about the storing of the yeasts (I think they can last years frozen).