consumer freezers are a bit too leathal for yeast.
Is the frost-free freezer that are bad due to the freeze-thaw cycles that keeps the frost-free.
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plactagonic@sopuli.xyz 3 days ago
How do you plan to freeze the yeast?
As I tried something like it few years ago I got that consumer freezers are a bit too leathal for yeast. I stored mine in tubes on agar in fridge and it was good after 4 months, but to get to that point was ~3 weeks of work.
ExcessShiv@lemmy.dbzer0.com 3 days ago
plactagonic@sopuli.xyz 3 days ago
I don’t remember that much, I think it is something about temperature - the -80°C freezers just freeze the culture much faster so you don’t kill the cells. You can mitigate it by using glycerol as antifreeze but it is bit more work.
With agar you store only the pure 1st gen of the yeasts so there is bit more work after you unfreeze them. So there is probably different method to freezing yeast cake.
When I messed with the yeast I used this playlist as instructions:
www.youtube.com/playlist?list=PLZ3Z2428mCTqDFK-3Z…
There is something about the storing of the yeasts (I think they can last years frozen).
poleslav@lemmy.world 3 days ago
I plan on following this process:
youtu.be/uxnUrDIqN5g
My girlfriend is a research scientist and she said they basically do the same thing but with a higher percentage of glycerine for their -80 degree freezers and liquid nitrogen so it should work well in a home freezer at the 25/75% ratio. One way to find out though lol