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alzymologist@sopuli.xyz â¨1⊠â¨week⊠ago
Haha, great to see success! Looks inviting to drink.
Be careful with propagation, without the 1-cell steps occasionally, itâs certain to get contaminated eventually. This particular strain does not even seem to have a killing feature to suppress other microbiota other than by starvation.
Yeah, the company is over and this was the last shipment. Actually the Posti lost the shipment and this was some informal sample I just made at home as a replacement long after operations stopped (so no fancy labels anymore!). Such an adventure.
But if someone needs the yeast, I still keep the library as a personal project, so DM.
robsteranium@lemmy.world â¨5⊠â¨days⊠ago
Could you elaborate on what you mean by â1-cellâ steps?
I didnât realise yeasts have âkilling featuresâ either⌠I thought the whole idea with fermentation was to create the right conditions for your favoured micro-organism to out compete the rest!
alzymologist@sopuli.xyz â¨5⊠â¨days⊠ago
The pure culture process (pretty much all the stuff invented by Pasteur) is based on isolating a single living cell of yeast and then growing a culture of its genetic clones by natural budding (our brewer yeast lost ability to reproduce by other means some centuries ago). This ensures that chances of mutation are small and there are no other organisms in the culture. It also rejuvenates yeast, as growth conditions in this process are much more favorable and pleasant, so that cells generation can build large energy reserves inside. This is surprisingly low tech, could be done at home (lol thatâs what I do), but it takes certain discipline, even more cleaning than brewing.
As of killing feature, Iâm not sure exactly what it is, but for some lines they report an ability to efficiently inhibit the activity of competing organisms by means others than just outcompeting them in multiplication and consumption of nutrients. These lines should be better for introducing uncooked stuff to fermentation (fruits, berries, spices, maybe raw honey), although Iâd say that really vigorous yeast can outcompete almost anything under common conditions, maybe save for really high pitch rate of acetobacter. I donât pay much attention to this feature really, but itâs a curiosity IMO.
robsteranium@lemmy.world â¨3⊠â¨days⊠ago
Can you really isolate a single cell without a laboratory?! What sort of equipment? This sounds more involved than just innoculating some wort with a spoonful of yeast sedimentâŚ
I tried to look at yeast under a 40x-640x microscope but just saw a brown blur! I presumed you would need expensive stains/ growth media to experiment properly.
plactagonic@sopuli.xyz â¨2⊠â¨days⊠ago
Itâs quite easy and microscope is unnecessary for this. Basically you spread it on petri dish and each dot (colony) originated from one cell.
This yt playlist goes quite deep in to it, you can follow it as instructions for yeast home lab.
www.youtube.com/playlist?list=PLZ3Z2428mCTqDFK-3ZâŚ